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Intrahepatic bacterial metataxonomic signature in non-alcoholic fatty liver disease

Mostrar el registro sencillo del ítem Sookoian, Silvia Salatino, Adrián Castaño, Gustavo Osvaldo Landa, María Silvia Fijalkowky, Cinthia Garaycoechea, Martín Pirola, Carlos José 2020-10-28T22:59:50Z 2020-10-28T22:59:50Z 2020-01-02
dc.description Fil: Garaycoechea, M. Hospital de Alta Complejidad en Red El Cruce Dr. Néstor C. Kirchner. Servicio de Centro Quirúrgico. Florencio Varela, Argentina. es_AR
dc.description.abstract Objective We aimed to characterise the liver tissue bacterial metataxonomic signature in two independent cohorts of patients with biopsy-proven non-alcoholic fatty liver disease (NAFLD) diagnosis, as differences in the host phenotypic features—from moderate to severe obesity—may be associated with significant changes in the microbial DNA profile. Design and methods Liver tissue samples from 116 individuals, comprising of 47 NAFLD overweight or moderately obese patients, 50 NAFLD morbidly obese patients elected for bariatric surgery and 19 controls, were analysed using high-throughput 16S rRNA gene sequencing. Results Liver bacterial DNA profile significantly differs between morbidly obese and non-morbidly obese patients with NAFLD. Bacteroidetes (p=1.8e-18) and Firmicutes (p=0.0044) were over-represented in morbidly obese patients and Proteobacteria (p=5.2e-10)—specifically Gammaproteobacteria and Alphaproteobacteria, and Deinococcus-Thermus (p=0.00012)—were over-represented in the non-morbidly obese cohort. Cohort-specific analysis of liver microbial DNA signatures shows patterns linked to obesity. The imbalance in Proteobacteria (Alpha or Gamma) among non-morbidly obese patients, and Peptostreptococcaceae, Verrucomicrobia, Actinobacteria and Gamma Proteobacteria DNA among morbidly obese patients was associated with histological severity. Decreased amounts of bacterial DNA from the Lachnospiraceae family were associated with more severe histological features. Proteobacteria DNA was consistently associated with lobular and portal inflammation scores. Microbial DNA composition corresponded to predicted functional differences. Conclusion This is the first comprehensive study showing that the liver tissue of NAFLD patients contains a diverse repertoire of bacterial DNA (up to 2.5×104 read counts). The liver metataxonomic signature may explain differences in the NAFLD pathogenic mechanisms as well as physiological functions of the host. es_AR
dc.language.iso en_US es_AR
dc.subject Enfermedad del Hígado Graso no Alcohólico es_AR
dc.subject Hígado Graso es_AR
dc.title Intrahepatic bacterial metataxonomic signature in non-alcoholic fatty liver disease es_AR
dc.type Article es_AR

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